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Author: Multiple Authors​

Citation: Multiple Authors. 2015. Temperature experiments.​

Publication Date: August 2015​

Slide 2: Temperature experiment: (A) Staphylococcus aureus (coagulase -), (B) Bacillus stearothermophilus, (C) Pseudomonas fluorescens and (D) Escherichia coli were grown on TSA for 24 hrs at varying incubator temperatures (4, 25, 40, and 60° C). Note: The 4°C plate was incubated for a week to allow for adequate Pseudomonas fluorescens growth. (Tasha Sturm, Cabrillo College, Aptos, CA)​

Slide 3: Temperature Experiment: Psychrophiles
​Psychrophiles are organisms capable of growth at low temperatures, typically from -5° to 15°C. (A) Staphylococcus aureus, (B) Bacillus stearothermophilus, (C) Pseudomonas fluorescens and (D) Escherichia coli were grown on TSA for 24 hrs at 4°C. Pseudomonas fluorescens was the only organism capable of growth at 4°C. (Tasha Sturm, Cabrillo College, Aptos, CA)​

Slide 4: Temperature Experiment: Psychrotrophs​
Psychrotrophs are organisms capable of growth at temperatures from -3° to 35° C. (A) Staphylococcus aureus, (B) Bacillus stearothermophilus, (C) Pseudomonas fluorescens and (D) Escherichia coli were grown on TSA for 24 hrs at 25°C. Staphylococcus aureus, Pseudomonas fluorescens and Escherichia coli were all able to grow at this temperature. No growth was seen for Bacillus stearothermophilus. Note: Although Staphylococcus aureus grew, it was not seen as confluent growth, i.e, there was not continuous growth along the streak line. (Tasha Sturm, Cabrillo College, Aptos, CA)​